Integration of Exogenous Genes Into the Genomes of Host Cells：Stable cell line construction means that exogenous genes enter the recipient cells and are integrated into the chromosomes, which enables host cells to express target genes for extended periods.
Site-Directed Insertion of Exogenous Genes into the Genomes of Host Cells：Cas9 systems disrupt the genome of the target region. By using the repair mechanism of the genome itself, exogenous genes (covering the two ends of the homologous arm) are inserted into the genome using homologous ends to produce a stable genetic transfer system.
Gene silencing in host cells: Pracgen offers a higher silencing efficiency by using electroporation Cas9 & gRNA mRNA technology for cells such as primary culture cells, stem cells, neurons, immune cells, and other cells.
① Integration of Exogenous Genes Into the Genomes of Host Cells
② Site-Directed Insertion of Exogenous Genes into the Genomes of Host Cells
③ Gene silencing in host cells
Tumor cell transformation / Humanization of mouse tumor cells
Monoclonal cell strains can be divided into several categories according to a range of possible downstream applications:
1. Monoclonal cell strains for research
2. Productive high-expression monoclonal cell strains for mass production of certain substances, for instance, proteins/antibodies
3. Effective targeting of cells for validation of cell therapy products