CRISPR-Cas9：The CRISPR-Cas9 gene editing system is a specific DNA modification technique for targeting genes. It combines the Cas9 protein with directed RNA, and uses the CRISPR complex to perform a precise location and cutting function, which can silence any single gene. Compared with ZFN and TALEN, CRISPR/Cas9 is easier to operate, more efficient, and can simultaneously introduce multiple mutations at different sites.
Pracgen now provides gene editing related services and Quick Edit CRISPR/Cas9 Cloning Kit .
1 Provide customized target gene knockout service
2 Provide customized sgRNA-Cas9 lentiviral vectors service
3. Provide target gene knockout stable cell lines service
4 Provide CRISPR screening service
5. Provide Quick Edit CRISPR-Cas9 Cloning Kit
FIG. 1 M is DNA Marker, - negative control, and + hsFOXR1 knockout cell pool
FIG. 2 M is DNA Marker, 1 hsFOXR1 knockout cell line, and 2 non-knockout control
FIG. 3 HsFOXR1 expression of A549 cell knockout gene detected by fluorescence quantitative PCR.
A hsGAPDH expression control
Group B hsFOXR1 knockout group expression. A549 non-knockout control group, A549 hsFOXR1 knockout group, negative control;
FIG. 4 Gene knockout sequencing result;
A549 HsFOXR1 gene sequencing result and A549 hsFOXR1 gene knockout sequencing result
Excellent technical team with proven track record;
Rich experience in gene editing projects;
Complete gene editing solution;
Super high gene editing efficiency ;
Quick Edit CRISPR-Cas9 Cloning Kit use pCAS9-sgRNA-puro vector construction & lentiviral vector packaging to simultaneously express spCas9, sgRNA and puromycin. It is also used for target gene knockout by cutting genomic DNA. Successful transfected / infected cells can be screened with puromycin to raise the rate of target gene knockout.
One-step to construct pCas9-sgRNA-puro vector
High positive rate
Simultaneous expression of Cas9 protein, sgRNA and puromycin
Directly used for transient experiments or lentiviral vector packaging
Storage conditions: 293T-GFP cell line stored at -80℃, the rest reagents stored at -20℃
Materials: Plasmid extraction kit, Self-provided puromycin, etc.
1. Designs gRNA and synthesis oligos;
2. sgRNA oligos anneal to form cohesive ends;
3. Linearized vector connected and transformed
4. Positive clone identification and sequencing;
5. Target gene knockout and identification;
1 easy to operate
2 High stability
3 Suitable for a variety of cells
4 Contain puro resistance for screening
5 Transient transfection, or lentiviral vector packaging
6 Include all major reagents